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題名:蓖麻凝集素之生理功能研究     (32點)
Studies on the Physiological Role of Lectin from Ricinus Communis
著者:王月雲(Yei-Yun Wang) ;楊冠政(K. J. Yang)
出版地區:台灣
出版城市:台北市
學科:生命科學
刊名:師大生物學報
卷期:14期(1979.10)
頁碼:51-66
語言:繁體中文
摘要: 中文摘要PDF ; 英文摘要PDF

崑殊護子( Ricinus com1伽nis. L. )去殼後,以碑磁盤j支持鼠,利用S e pha ro s e 4B視和|生國拉色層分析, 以0.2 M Galact ose i中溶洗出而取得崑扉植物凝集素(Ricinus communis agglutinin 簡寫為R.C.A ) ,再經5叩ha d ex G -100分離而得二種蛋白質,分別標記為R CA - I & RCA糊口° RCA- I 對血球具有善于益的凝祟能力, 而RCA- IT 對小白鼠( 19 士19 )有致死作用,其LD 已。這已0 ‧ 03 mg/峙。.;f等新鮮麓爆發子播種在湖溼蛙k中,直於3 0 ° C, 12 小時光照, 光鎮ia 6∞o lux 的植物培接箱內,經過2 1夭的培養,每天澆以Hoa gl and ' s :£企液,取一遇內梭棒幼苗及9, 11 , 14 ,19 及21 天的植林區分為祿、莖, "1- 等三部分抽取純化, 主主定量RCA 含量, 利用電泳、凝血能力,動物毒性及免疫反應等方法研究龍應種子發芽過程中RC A的分佈及消長情形。結果顯示生長第三天時,其R CA令量已i成為原來的三分之一,至第七天只剩下十分之一,科用提捧該廠不論在祿、莖、華等部位均含有R CA封第九天後,萎縮駐扎尚存留一些,但祿、笠、華等處R CA完企消失。於培養第十四天後,本論越祿的任何部位均永找到R CA存在,再經S e phad位G - I 00分析知R C A - ll 比RCA - I遞減得更快經毒性試驗得相同結果,由電泳及免疫擴散反應,確認植~*抽取所得R CA余種子內所得相同。 將R C A,為母珠凝1美索( Abrin) 及Concavalin A 分別作封常見的良真萬及土垠細菌生長的影響多利用挖洞法發琨R CA對某些鹿三ι苗及是表性生長的植物為原苗布抑制作用。尤其R CA對某些不完全蔔絨的農商如Fu s a r i um sola師, As戶ergillus Ii scheri ,Mucor rouxii 等有明顯抑制作用, 而RC A 對Peni cil Zi紹, C ladosporium , Rhi zoto削G抑制較差,當1 1者見酵母菌沒布抑制作用。據分析這些能被R CA抑制的真笛,其茵絲細胞壁構造均為葡:&j糖果合物(glucan )或幾丁噴( ch i ti n )組成的。為母~凝捧素對土垠真揖抑制較均,而C onA 重p使將濃度提高至7 mg/ml 仍無抑制作用。 利用F il t e r dish 方法梭查十五種常見的土垠細昔, 發現凝祟素對細首都沒有抑制作用。由土結果推iQ1越物體種子內今凝霉素,有些除了對動物具有致死及係護繁衍之用,寫些豆科植物授主長素具有選擇其共生根瘤茵的專一性,本砂T克證明了提集素對土垠微生物有條道機制,尤其對土垠臭萬扮演了一種防衛角色。特別是當橙子早期埋於土垠內發生浸潤作用,種子膨脹及萌芽過程中,袋子本身可時禦為原茵之侵入。當植物新生。t:長~能進行光合作用,不必仲賴極尋L供給養分時對ft'r永病原拭抗力加強,也并不再需要提宇晨素的係護,則提j長索於越林體內終告消失3這將待進一步證實。
Castor bean (Ricinus communis. L ) seeds were peeled and extracted with buffer saline solution, using sepharose-4B affinity chromatography & eluting the with 0.2M D-Galactose. We obtained Ricinus communis agglutinin, abbreviated as Then was passed through Sephadex G-100, which was washed with P.B.S, if was into two proteins, they were marked to be RCA- I & RCA-IT. RCA- I had agglutinated the R.B.C cell, but RCA-II had lethal dose response with mice Cl9±1g), its LD50= mg jkg body. Freshed castor bean seeds were fertilized and soaked overnight in running water & germinated in moist vermiculite. 'Then put into the growth chamber which was 30 DC, 12 hr light, light intensity was 6000 lux. During the plant grew, we gave the with Hoagland's solution everyday. Collected its Ist weekly seedling and‧ the plants of 9, 11, 14.& 21 days and divided it into root, stem, leaf, then extracted and purified. According to Lowry method, we determined the lectin content, with electrophoresis, Hemagglutination ability, animal toxicity & immunoreaction etc; we studied the successation& distribution of R.C.A. during germination. Results showed that the third day 'growth, the R. C.A content droped down to original one third. After it grew seven days, it became one tenth. With Hemagglutination test, we discovered that even at the 'site of root, stem, leaf had the R.C-A. But at the nine day growth, only endosperm had a little where in root, stem, leaf which had no lectin. However after 14 day growth; any site of plant had no lectin existed. Byi passing sephadex G-100 chromatography, we knew that RCA-IT was lost more quickly than RCA-I, using animal toxicity test had the same result. By Electrophoresis & Immunology reaction, we identify that the lectin from plant extracts was the same as seeds. Using RC.A, Abrin & ConA, treated on general soil fungi & soil bacteria growth. We used cut-plate method & di scovered iRi Cc.A, inhibited some saprophyte & faculative growth plant pathogen, especially RC.A. would effect to some imperfect fungi, such as Fusarium solani, Aspergillus fi scheri , Murco rouxii etc, but have a little effect on Penicillum, Cladosporium, Rhizotonia, however R.C.A have no inhibition to yeast growth. Abrin effected to soil fungi, but ConA concentration was 7mgjml. showed no inhibition, if ConAconc was 10mg /ml had a little inhibition to Fusarium solani . According to this data, analyzed this structure of fungi cell wall which could be inhibited; that was gluean or chitin cpd. & proved R.C.A. inhibited hyphae extension.


    

本卷期目次
師大生物學報 14期 (1979.10)
剝奪大白鼠反常睡眠對睡眠型式之影響/ 李蘊香吳京一
毛黴菌目之分離、培養與觀察-一種針刺弓黴菌(Absidia spinosa)之研究/ 何小曼簡秋源
IN VIVO EFFECT OF TAURINE ON CALCIUM FLUX OF HEART DURING EPINEPHRINE-INDUCED ARRHYTHMIAS/ Jinn-Ding Lin
淡水河流域水生物調查及水質等級評估/ 洪正中
五股蘆洲沼澤地區之鳥類相/ 呂光洋蔡航椰
兩種裂頭條蟲(闊節裂頭條蟲和曼氏裂頭條蟲)蟲卵的掃描電子顯微研究/ 溫永福鄭湧涇蔡在壽
蓖麻凝集素之生理功能研究/ 王月雲楊冠政
扇平山區動物採集報告/ 生物學系68年度暑假動物採集隊
 
   
 
   

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