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題名:蕹菜萌發期間澱粉及蛋白分解?基因表現之組織特異性     (24點)
Tissue Specific Expression of a-amylase and Proteinase Genes in Germinating Water Spinch Seeds (Ipomoea aquatica Forsk)
著者:侯新龍(Shin-Lon Ho) ;林聰慧(Tsung-Hui Lin) ;張永達(Yung-Ta Chang) ;余淑美(Su-May Yu) ;童武夫(Wu-Fu Tong)
出版地區:台灣
出版城市:台北市
學科:生命科學
關鍵字:種子萌發 ; 澱粉分解? ; 蛋白分解? ; 基因表現 ; Seed germination ; α-amylase ; Proteinase ; Gene expression
刊名:師大生物學報
卷期:34卷2期(1999.12)
頁碼:105-116
語言:繁體中文
摘要: 中文摘要PDF ; 英文摘要PDF

蘿菜萌發期間稜于內的不同組織梅基因表現之差異,與生、理機能之關聯性為本文之研究重點o 萌發0 . 5 天種子內的胚乳, 可測到相當高的JE粉分解梅( 如a myl ase ) 法性及大量澱粉分解晦m到吋A 的堆積, 但隨後都急速下降ι 顯然這些鷗分子及其mRNA' 很可能是在種子成熟時期所轉錄、轉譯的o而萌發期間于莘及舵、軸祁j則不到嚴粉分解梅mRNA的存在,因此推論澱粉分解酪基因在此萌發期間,無論是胚乳、子幸成胚軸組織皆~表現。蛋白分解梅( proteinase) mRNA 在胚乳及胚軸組織,也是隨著殺子的萌發而急速下降、消失o唯有子黨組織的晦基因在萌發期間有所表稅。 細胞分裂素( benzy ladeni ne )和吉貝素( gibberellic acid) 的處理, 並不影響胚乳組織恥amylase的活性。細胞分裂素則對于萃的蛋白分解醜活性有短暫性的促進效果,此促進作用似乎與于萃的生長發育有關o從南方轉潰分析結果顯示, a-amylase 及蛋白分解梅瑟困在基因組中皆為單份( single copy) 基因o
Differential expression of a-amylase and proteinase genes in relation to the physiological function of different tissues in the seed of water spinach during germination were studied. High levels of a-amylase activity and its mRNA were detected in the endosperm of 0.5 day germinating seeds, but both of them decreased rapidly during germination. This enzyme and its mRNA are most likely to have been produced during seed maturation. Unlike endosperm, a-amylase mRNA was not detectable in either cotyledon or embryo axis during seed germination. These results indicate that the a-amylase genes are repressed in all tissues of water spinach seed during the observed period of germination. Similarily, proteinase mRNAs in the tissues of endosperm and embryo axis also decrease rapidly during germination, although the proteinase gene expresses in the germinating cotyledon. The activities of a-amylase in endosperm and cotyledon were not affected by treatment of either benzyladenine or gibberellic acid. A temporary increase in proteinase activity was observed by treatment with benzyladenine in germinating cotyledon which might be concerned with the growth and development of the tissue. Southern blot analysis indicates that the genome may contain a single copy for both aamylase and proteinase genes.




本卷期目次
師大生物學報 34卷2期 (1999.12)
蕹菜萌發期間澱粉及蛋白分解?基因表現之組織特異性/ 侯新龍林聰慧張永達余淑美童武夫
第三B型黏多醣儲積症(MPS ⅢB):NAGLU基因的突變分析/ 林秀珠林炫沛李桂楨
影響蛇類跨越能力的變因/ 林郁君黃基礎杜銘章
利用trnL intron序列分析低等金縷梅類植物分子親緣/ 吳瑞蛾童武夫王震哲
墾丁國家公園台灣梅花鹿(Cervus nippon taiouanus)磨樹及其對當地林木之影響/ 陳順其王穎
臺灣產褸網蜘蛛分子遺傳結構之分析/ 林俊宏李錦年楊智傑呂柏緯陳世煌呂光洋方剛
 
   
 
   

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