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題名:Correlation of Elevated mRNA Expression of p53 Gene and Repair Genes with Lung Cancer Chemoresistance     (22點)
p53抑癌基因與四種DNA修補基因其mRNA表現量增加與肺癌病人化療抗藥性之相關性研究
著者:Pei-Jung Lee(李佩蓉) ;Yng-Ju Liang(梁瑩如) ;Chuen-Ming Shih(施純明) ;Yi-Ching Wang(王憶卿)
出版地區:台灣
出版城市:台北市
學科:生命科學
關鍵字:p53抑癌基因 ; DNA修補基因 ; 反轉錄聚合酵素反應 ; 肺癌 ; 抗藥性 ; p53 gene ; Repair gene ; RT-PCR ; Lung cancer ; Chemoresistance
刊名:Biological Bulletin of National Taiwan Normal University
卷期:35卷2期(2000.12)
頁碼:95-105
語言:英語
摘要: 中文摘要PDF ; 英文摘要PDF

肺癌是國人最重要的痞症致死原因,大部分接受放射線治療及化學治療的肺痞患者,常常因產生抗性而治療失敗,因此了解瘤細胞抗藥性之機制對於發展更有效的治療將有莫大的幫助。已有文獻指出p 5 3 4Cp癌基因與數種DNA 修補基因其mRNA表現增加與痞症舟人化療抗禁性具有正相關,所以本研究以定量同步反轉錄聚合酵素反應( quantitative multiplex reverse transcription-polymerasechain reaction) 同時檢測p5 3 抑痞基因與四種DNA 修補基因XPCC,的iSH2, XRCCl , 與ERCCl的表現量;為了瞭解這些基因的mRNA表現是否能作為肺癌病人治療效泉的監控指標,此定量同步反轉錄聚合酵素分析,以1 2位具有化療抗藥性的肺痞病人與1 1 位治療有效的病人作比較分析。在改良過的定量同步反轉錄聚合酵素反應中,顯示p53抑癌基因與四種修補基因分別在放大2 6- 3 2與3 0- 34個循環數時,會呈王見線性關f系,因此利用此反應條件,分析肺癌病人之p 53抑痞基因與四種修補基因mRNA的表現量與抗藥性的相關性。結泉顯示,其化療抗藥性的病人其p53抑痞基因的平均表現量為O.肘,化療無效的病人為0 . 2 8 '此差異有接近顯著的統計分析趨勢( P = 0 . 07 )。本研究結果推測, p53 基囚的mRNA 表現量的提高或許可以成烏肺嘉病人化療抗藥性的預測指標。
Lung cancer is the leading cause of cancer death in Taiwan. In the chemotherapeutic modalities used to treat the lung cancer, the great majority of patients will relapse with tumor that is largely resistant to further treatment. In addition, clinical and experimental studies suggest that alteration of the expression level of the p53 gene and repair genes may be associated with chemoresistance. Therefore, we evaluated the differences in the expression levels ofp53 gene and four repair genes, including XPCC, hMSH2, XRCCI, and ERCC1, in 12 chemo-resistant lung cancer patients and 11 chemo-sensitive lung cancer patients using an optimized quantitative multiplex reverse transcriptase-polymerase chain reaction (RT-PCR). The modification experiments of the quantitative multiplex RT-PCR showed that amplification was linear between 26-32 cycles and 30-34 cycles for the analyses of the p53 gene and repair genes, respectively. We used this optimized quantitative multiplex RT-PCR to analyze the correlation of mRNA expression of the p53 gene and the repair genes with chemo-resistance of lung cancer. The data indicated that the mean mRNA level of the p53 gene in chemo-resistant patients (0.65) was 132.1 % higher than that of the chemo-sensitive patients (0.28). The chemo-resistant cases had an almost significantly higher (P=0.07) mean expression level of the p53 gene than did the effective cases. Our results suggest that an elevated p53 mRNA level may predict the chemoresistance of lung cancer patients.


    

本卷期目次
Biological Bulletin of National Taiwan Normal University 35卷2期 (2000.12)
台灣地區草蜥類生物地理類緣關係之研究/ 高善呂光洋
臺灣五位胰島素阻抗性症候群患者的胰島素受器變異分析/ 楊喬雯林炫沛張心怡李桂楨
重組第一類轉形生長因子與篦麻毒素A融合蛋白質對人類癌症細胞之生長抑制效應/ 陳詩蕙康淵琳邱建智方剛
瓦拉米地區台灣山羌之活動模式/ 陳怡君王穎
Notes on Commelinaceae of Taiwan: Cyanotis and Belosynapsis, and the rediscovery of Murdannia edulis/ Ching-I PengYo-Jiun ChenJenn-Che Wang
Correlation of Elevated mRNA Expression of p53 Gene and Repair Genes with Lung Cancer Chemoresistance/ Pei-Jung LeeYng-Ju LiangChuen-Ming ShihYi-Ching Wang
 
   
 
   

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