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1
題名:CHILLING STRESS EFFECTS ON THE GROWTH, MITOCHONDRIAL ACTIVITY AND PROTEIN SYNTHESIS IN ETIOLATED MUNGBEAN SEEDLINGS    
低溫逆境對綠豆白化幼苗之生長、粒線體活性及其蛋白質合成之影響
著者:Yih-Ming Chen(陳益明) ;Hsin-Fei Liu(劉心斐) ;Chu-Yung Lin(林秋榮)
出版地區:台灣
出版城市:台北市
學科:生命科學
刊名:Taiwania
卷期:36卷3期(1991.9)
頁碼:277-290
語言:英語
摘要: 中文摘要PDF ; 英文摘要PDF

本研究此對低溫敏感的植物-綠豆(Vigna radiate L.)?材料。在黑暗中萌芽32小時的綠豆幼苗,經4℃處理不同時間後,分析其生長、粒線體活性及蛋白質合成能力等;其結果顯示下列各種現象接受到抑制:(一)幼苗的生長,(二)粒線體的succinate dehydrogenease活性;(三)幼苗的蛋白質合成能力。但低溫處理會導致幼苗在室溫下呼吸速率增快,且幼苗的組織也受到傷害,因此組織內溶質的漏出物增加。在4℃低溫處理下所合成的35S-蛋白質,經SDS-膠體電泳分析及fluorography後,其中有13種蛋白質帶(protein band)是低溫誘導出的蛋白質(chilling induced proteins);其分子量是110, 88, 78, 69, 58, 48, 38, 27, 22, 19, 14, 12.5和10.5KD,其中88, 69, 58, 48, 38, 14, 12.5 and 10.5KD是主要的低溫誘導蛋白質。這些蛋白質完全與綠豆的熱休克蛋白質不同。
Mungbean (Vigna radiate L.), a chilling sensitive plant species, was used in this study. Seeds were germinated at 28℃ in the dark for 32 h before cold treatment. The seedlings were chilled at 4℃ for various durations. Growth, respiration rate, protein synthetic activity, cellular leakages and succinate dehydrogenase activity in isolated mitochondria were then determined. Chilling at 4℃ suppressed seedling growth, increased solute leakages, suppressed succinate dehydrogenase activity in isolated mitochondria, inhibited general protein synthesis and induced the synthesis of a set of proteins termed chilling-induced proteins. The molecular weights of these chilling-induced proteins were 110, 88, 78, 69, 58, 48, 38, 27, 22, 19, 14, 12.5 and 10.5Kd, respectively and the proteins with molecular weights of 88, 69, 58, 48, 38, 14, 12.5 and 10.5Kd were the major chilling-induced proteins of mungbean seedlings. These chilling-induced proteins were different from the heat shock proteins of mungbean seedlings.


    

本卷期目次
Taiwania 36卷3期 (1991.9)
ALLELOPATHIC POTENTIALS OF A WILD RICE, ORYZA PERENNIS/ Chang-Hung ChouF. J. ChangH. I. Oka
THERMOPHILIC AND THERMOTOLERANT FUNGI IN TAIWAN (III)/ Guey-Yuh ChenZuei-Ching Chen
CHLOROPHYLL FLUORESCENCE AS AN INDICATOR TO DETECT DIFFERENTIAL TOLERANCE OF SNAPBEAN CULTIVARS IN RESPONSE TO O3 STRESS/ Edward H. Lee
THE STUDY ON AERIAL ROOT OF FICUS MICROCARPA L. f. (1) The structure of root cap/ Ling-Long Kuo-Huang
CORTICOLOUS MYXOMYCETES OF TAIWAN: ON THE BARK OF PINUS TREES FROM CENTRAL AND NORTHERN TAIWAN/ Yu-Chung ChiangChin-Hui Liu
MUSHROOM FLOLA OF LAN-YU ISLAND (1)/ Kai-Wun YehZuei-Ching Chen
NOTES ON THE FLORA OF TAIWAN (12) The Caesalpinia L. (Leguminosae-Caesalpinioideae)/ Shing-Fan HuangTseng-Chieng Huang
CHILLING STRESS EFFECTS ON THE GROWTH, MITOCHONDRIAL ACTIVITY AND PROTEIN SYNTHESIS IN ETIOLATED MUNGBEAN SEEDLINGS/ Yih-Ming ChenHsin-Fei LiuChu-Yung Lin
 
   
 
   

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