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題名:RAPD analysis of Lycium barbarum medicine in Taiwan market     (8點)
以逢機擴增多型性DNA分析市售枸杞藥材之遺傳變異
著者:Kur-Ta Cheng(鄭可大) ;Hsien-Chang Chang(張憲昌) ;Her Huang(黃河) ;Cher-Tong Lin(林哲堂)
出版地區:台灣
出版城市:台北市
學科:生命科學
關鍵字:枸杞 ; 逢機擴增多型性DNA ; Chinese medicine ; DNA ; Lycium barbarum ; RAPD
刊名:Botanical Bulletin of Academia Sinica
頁碼:11-14
語言:英語
摘要: 中文摘要PDF ; 英文摘要PDF

本研究以逢要擴增多型性DNA(RAPD)技術,分析二十家中藥店市售枸杞藥材之遺傳變異。以六十條含十個逢機排列之核±O酸的引子,進行RAPD分析的結果顯示,有四條引子的反應產物,可將樣品呈現不同之電泳圖譜。此四條引子分別為OPD-15, OPG-15, OPT-12和OPT-17。其結果均一致,在二十個市售樣品中,有十五家為第一型,另五家為第二型。該研究顯示,市售枸杞藥材的遺傳變異低,推測這些藥材可能來自兩個不同的來源,或根本為兩個不同的種,若有標準品,即可進一步證實。
In this study, we investigated Lycium barbarum, a Chinese medicine sold on the Taiwan market, using RAPD analysis. Sixty oligonucleotide primers were used to screen twenty randomly selected samples in the analysis. Total DNA extracted from the fruit of the medicine material was used as template in the PCR reaction. Four primers: OPD-15, OPG-15, OPT-12 and OPT-17, showed distinct polymorphic patterns, but others exhibited profiles nearly identical to the other samples used in the study. We found that only two RAPD fingerprinting types of these primers were outlined from twenty collected Lycium samples. Fifteen samples showed the first type of profiles while only five samples resulted of the second type. A low genetic diversity among the Lycium barbarum samples was revealed by RAPD analysis.


    

本卷期目次
Botanical Bulletin of Academia Sinica
Construction and evaluation of transgenic tobacco plants expressing the coat protein gene of papaya ringspot virus with different translation leaders/ Ying-Huey ChengShyi-Dong Yeh
RAPD analysis of Lycium barbarum medicine in Taiwan market/ Kur-Ta ChengHsien-Chang ChangHer HuangCher-Tong Lin
Low RAPD polymorphism in Archangiopteris itoi, a rare and endemic fern in Taiwan/ Tsai-Wen HsuShan-Juei MooreTzen-Yuh Chiang
Phosphate starvation induction of acid phosphatase in Ulva lactuca L. (Ulvales, Chlorophyta)/ Tse-Min Lee
Effect of NaC1 on germination, growth, and soluble sugar content in Chenopodium quinoa Wilid. Seeds/ Fernando E. PradoCecilia BoeroMiriam GallardoJuan A. Gonzalez
Drought stress effects on water relations of wheat/ M. R. B. SiddiqueA. HamidM. S. Islam
Polyunsaturated fatty acid production with Mortierella alpine by solid substrate fermentation/ Hung-Der JangYuh-Yih LinShang-Shyng Yang
A formulated container medium suppressive to Rhizoctonia damping-off of cabbage/ Jenn-Wen HuangHung-Chang Huang
The sexual stage of Phytophthora polygoni Saw./ X. B. ZhengH. H. Ho
Long-term ecological research in the Yuanyang Lake forest ecosystem I. Vegetation composition and analysis4/ Chang-Hung ChouTze-Ying ChenChi-Cheng LiaoChing-I Peng
Some chytrids of Taiwan (Ⅲ)/ Shu-Fen ChenMei-Lien HsuChiu-Yuan Chien
A new species of Lambdasporium from Taiwan/ J. L. ChenW. S. LinS. S. Tzean
 
   
 
   

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