原名「台灣學術線上」
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題名:Adventitious shoot regeneration from stem internode explants of Adenophora triphylla (Thunb.) A. DC. (Campanulaceae)-an important medicinal herb     (14點)
輪葉沙參莖段培植體誘導不定芽再生
著者:Chung-Chuan Chen(陳忠川) ;Suh-Jau Chen(陳素昭) ;Hsin-Sheng Tsay(蔡新聲)
出版地區:台灣
出版城市:台北市
學科:生命科學
關鍵字:輪葉沙參 ; 桔梗科 ; Denovo再生 ; 種源保存 ; 中藥材 ; 微體繁殖 ; 沙參 ; Adenophora triphylla (Thunb.) A. DC. ; Campanulaceae ; De novo regeneration ; Germplasm conservation ; Medicinal herb ; Micropropagation ; Sha shen
刊名:Botanical Bulletin of Academia Sinica
頁碼:1-7
語言:英語
摘要: 中文摘要PDF ; 英文摘要PDF

輪葉沙參(桔梗科)如載於神農本草經列為上品,自古即用於鎮咳、去痰及排膿消腫,近代藥理分析更發現具有降血壓的效用,是一種相當重要且值得研究開發的中藥材。試驗結果顯示,取自上、中、下不同節位的培植體和生長調節劑深度會影響再生作用的進行;將莖段培植體培養於MS基本鹽類添加2.22~35.51μM BA及0.45μM NAA的培養基可成功誘導不定芽再生;此種培植體如繼代培養於MS基本鹽類添加17.75μM BA培養基時,能獲得大量增生的不定芽。當萌生的新芽培養於含1/4MS基本鹽類添加5.37μM NAA培養基則有促進根系生長的效果;已著生根系的瓶苗移出種植於土壤質並置於生長箱中馴化栽培,總計自節間培植體開始培養至馴化後獲得完整的苗株,需時約6~7個月,據此可建立一套有效率的繁殖模式。
Efficient plant regeneration system has been established from stem internode explants of Adenophora triphylla (Thunb.) A. DC. (Campanulaceae), an important medicinal herb. Adventitious shoots were induced by culturing the stem internode explants on Murashige and Skoog (MS) basal medium supplemented with 2.22-35.51 μM N6-benzylaminopurine (BA) in combination with 0.54 μM α-naphthaleneacetic acid (NAA). The regeneration potential varied with the developmental stage of the stem explant and growth regulator combination. Adventitious shoots formed a new crop of multiple shoots when subcultured on MS medium supplemented with 17.75 μM BA. Shoots were rooted on 1/4-strength MS basal medium supplemented with 5.37 μM NAA. Plantlets produced from shoots were transferred to soil and acclimatized in a growth chamber. Plants appearing morphologically normal were obtained six to seven months after culture of stem internode explants.




本卷期目次
Botanical Bulletin of Academia Sinica
Adventitious shoot regeneration from stem internode explants of Adenophora triphylla (Thunb.) A. DC. (Campanulaceae)-an important medicinal herb/ Chung-Chuan ChenSuh-Jau ChenHsin-Sheng Tsay
Characteristics of the acidic environment of the Yuanyang Lake (Taiwan)/ Jiunn-Tzong WuShih-Chieh ChangYun-Sen WangYu-Fa WangMing-Kuang Hsu
Purification and characterization of glutamine synthetase from the unicellular cyanobacterium Synechococcus RF-1/ Hso-Freng YuanChiung-Min WangHan-Wei Kung
Vase life Eustoma grandiflorum as affected by aluminum sulfate/ Li-Jen LiaoYu-Han LinKuang-Liang HuangWen-Shaw Chen
Detection of leaf-associated fungi based on PCR and nucleotide sequence of the ribosomal internal transcribed spacer (ITS) in Miscanthus/ Yu-Chung ChiangChang-Hung ChouPei-Ray LeeTzen-Yuh Chiang
Mechanism of host cell death induced by infection of Escherichia coli with the c2 clear-plaque mutant of phage f1/ Shan-Hua LinWen-Ping ChenTsong-Tsong Kuo
Cold-acclimation and root temperature protection from chilling injury in chilling-sensitive mungbean (Vigna radiata L.) seedlings/ Mon-Yaw ChangSwu-Ling ChenChung-Fen LeeYih-Ming Chen
Karyomorphology of Biebersteinia Stephan (Geraniaceae) and its systematic and taxonomic significance/ Jian-Quan LiuTing-Nong HoShi-Long ChenAn-Min Lu
The genus Tubocapsicum (Solanaceae)/ Zhi-Yun ZhangChing-I Peng
High frequency somatic embryogenesis and plant regeneration of an elite Chinese cotton variety/ Bao-Hong ZhangRong FengFang LiuQing-Lian Wang
 
   
 
   

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