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題名:Isolation and characterization of the third gene encoding a 16.9 kDa class I low-molecular-mass heat shock protein, Oshsp 16.9C, in rice     (16點)
水稻中第三個16.9 kDa第一族低分子量熱休克蛋白質基因,Oshsp 16.9C,之分離與鑑定
著者:Pi-Fang Linda Chang(張碧芳) ;Chung-Yi Huang(黃仲義) ;Fa-Cheng Chang(張法正) ;Tong-Seung Tseng(曾冬筍) ;Wan-Chi Lin(林婉琦) ;Chu-Yung Lin(林秋榮)
出版地區:台灣
出版城市:台北市
學科:生命科學
關鍵字:熱休克蛋白質 ; 熱休克基因 ; 低分子量熱休克蛋白質 ; 水稻 ; Heat shock protein ; Heat shock gene ; Low-molecular-mass heat shock protein ; Oryza sativa L
刊名:Botanical Bulletin of Academia Sinica
頁碼:85-92
語言:英語
摘要: 中文摘要PDF ; 英文摘要PDF

利用水稻16.9 kDa熱休克蛋白質(HSP)基因之cDNA,pTS1,作為南方墨點分析法之探針,可在以Eco RI限制切割之水稻基因組DNA樣品中,偵測到五個清晰的條帶,其大小分別為9.5、5.9、3.4、2.5及1.7 kb,經過本研究室之分析,其中包含六個不同的基因。將5.9 kb之DNA片段中可能包含一低分子量熱休克蛋白質基因。經由定序分析此3.5 kb片段之核酸序列,推測其可能之轉譯產物為包含149個氨基酸之16.9 kDa熱休克蛋白質,其等電點(PI)值估計為6.42。此基因和本研究室已發表的二個水稻第一族低分子量熱休克蛋白質基因,Oshsp 16.9A及Oshsp 16.9B,在轉譯區內之核酸序列具有相當高的同質性,分別有93.1%及94.3%的相同度。此基因之氨基酸推演序列和Oshp 16.9A及Oshsp 16.9B基因幾乎完全相同,分別只有11個及10個氨基酸之不同,因此將此基因定為Oshsp 16.9C(accession number: U81385)。已知水稻第一族低分子量熱休克蛋白質基因之3'非轉譯區(3' UTR)的核酸序列相似性較低,因此我們將Oshsp 16.9C之3'非轉譯區作為探針,以分析其基因之表現情形。
Using rice cDNA pTS1 encoding a 16.9 kDa heat shock protein (HSP) as a probe for Southern hybridization analysis, we observed five prominent bands of 9.5, 5.9, 3.4, 2.5, and 1.7 kb in the Eco RI digests of rice genomic DNA and found them to contain six individual genes. The 5.9 kb DNA fragment was further digested with Hind III to generate three fragments of 3.5, 1.7 and 0.7 kb, and we found, using pTS1 cDNA as a probe, the 3.5 kb fragment contained a putative low-molecular-mass (LMM) HSP gene. The DNA sequencing of 3.5 kb fragment revealed encoding of a presumptive 16.9 kDa HSP (149 amino acid residues) with predicted pI value of 6.42. The nucleotide sequence of this gene was highly homologous to the coding regions of two rice class I LMM HSP genes, Oshsp 16.9A and Oshsp l6.9B, sharing 93.1% and 94.3% sequence identity, respectively, as published previously in our laboratory. The deduced amino acid sequence of this gene is similar to those of the Oshsp l6.9A and Oshsp l6.9B genes with a difference of only 11 and 10 amino acids, respectively. It was hence designated as Oshsp l6.9C (accession no. U81385). We used 3' UTRs (untranslated regions) for analysis of Oshsp l6.9C gene expression since the 3' UTRs of these three genes showed very low sequence homology.


    

本卷期目次
Botanical Bulletin of Academia Sinica
Identification of Chlorella spp. isolates using ribosomal DNA sequences/ Luen HwuChiou-Jau ChoS. S. TzeanzAlan Lin
Purification and characterization of sucrose phosphate synthase from sweet potato tuberous roots/ Wen-Hui WeiRui QinYun-Chun SongLe-Qun GuoMing-Guang Gu
Identification of Chlorella spp. isolates using ribosomal DNA sequences/ Hsiuan-Lin WuRuey-Shyang HseuLiang-Ping Lin
Purification and characterization of sucrose phosphate synthase from sweet potato tuberous roots/ Wei-Liang ChenDong-Jiann HuangPang-His LiuHeng-Long WangJong-Ching SuPing-Du Lee
Effects of ammonium, phosphate, and salinity on growth, gas exchange characteristics, and ionic contents of seedlings of mangrove Kandelia candel (L.) Druce/ Yuan-Hsun HwangShuh-Chun Chen
Existence analysis of populations of Mosla hangchowensis, an endangered plant/ Ying GeJie Chang
Helicoon doliiformis sp. nov. and two similar helicosporous hyphomycetes form Taiwan/ H. S. Chang
Lagenandra keralensis (Araceae), a remarkable new species from India/ M. SivadasanV. Abdul JaleelBobby Thomas
Isolation and characterization of the third gene encoding a 16.9 kDa class I low-molecular-mass heat shock protein, Oshsp 16.9C, in rice/ Pi-Fang Linda ChangChung-Yi HuangFa-Cheng ChangTong-Seung TsengWan-Chi LinChu-Yung Lin
An assessment of genetic relationships in cultivated tea clones and native wild tea in Taiwan using RAPD and ISSR markers/ Jou-Ann LaiWei-Chen YangJu-Ying Hsiao
 
   
 
   

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