原名「台灣學術線上」
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1
題名:Ultrastructural study and lipid formation of Isochrysis sp. CCMP1324     (16點)
等鞭金藻的脂質形成過程與超微結構之研究
著者:Ching-Piao Liu(劉清標) ;Liang-Ping Lin(林良平)
出版地區:台灣
出版城市:台北市
學科:生命科學
關鍵字:廿二碳六烯酸 ; 等鞭金藻 ; 油脂形成 ; 多元不飽和脂肪酸 ; 超微結構 ; Docosahexaenoic acid ; Isochrysis sp. ; Lipid formation ; Polyunsaturated fatty acids (PUFA) ; Ultrastructure
刊名:Botanical Bulletin of Academia Sinica
頁碼:207-214
語言:英語
摘要: 中文摘要PDF ; 英文摘要PDF

利用光學及穿透式電子顯微鏡技術,觀察海洋微藻Isochrysis sp. CCMP1324其形態與脂肪形成之過程,發現其脂肪含有多元不飽和脂肪酸,尤其是廿二碳六烯酸(DHA),為時下流行之健康食品,對於人體健康維護及水?養殖餌料,提供相當大之助益。在10% CO2之通氣及10 klux之連續照明下,細胞中廿二碳六烯酸之含量繼續增加至八天之培養,最終含量為每升含16毫克。實驗中並探討基脂肪在生長過程之形成與變化,為首次利用電顯探討等鞭金藻油脂形成之研究報告。
This study investigates methods for extracting lipids from microalgae and analyzes the effects of culture media as well as culture conditions on PUFA yields and total fatty acid contents. Experimental results of an optimal culturing of Isochrysis spp. were based on a 3.2% salinity culture medium. These microalgae were cultured in a 1-2 L Roux's flat-flask and a 5L jar fermentor. The optimum culture temperature and initial pH for DHA production were 25℃ and 8.0, respectively. Pigments included chlorophylls a and c. The DHA yield increased with cultivation time until the eighth day. Optimum DHA amounts in the cells were reached under aeration with 10% CO2 and with continuous illumination of 10 klux. The biomass dry weight reached 4g per liter of culture, and the DHA production reached 16 mg per liter of culture. Lipid bodies in Isochrysis spp. and related genera were observed during culture by light and transmission electron microscopy; 0.5~3.0 pm sized lipid bodies were confirmed by staining with Sudan Black B in cells from log stage to stationary stage cultures. These results demonstrated that DHA-containing lipid bodies in cells can be produced and accumulated in marine Isochrysis spp.


    

本卷期目次
Botanical Bulletin of Academia Sinica
Characterization of a 10 cM region of rice chromosome 5/ Teh-Yuan ChowYa-Ting ChaoSu-Mei LiuHong-Pang WuMu-Kuei ChuChing-San ChenYue-Ied. Hsing
Mapping fertility-restoring genes of rice WA cytoplasmic male sterility using SSLP markers/ Run-Chun JingXiao-Ming LiPing YiYing-Guo Zhu
RAPD variation in relation to population differentiation of Chamaecyparis formosensis and Chamaecyparis taiwanensis/ Shih-Ying HwangHuei-Wen LinYi-Shou KuoTsan-Piao Lin
Molecular phylogeny of nitrogen-fixing unicellular cyanobacteria/ Tan-Chi HuangShu-Miaw Chaw
Rhododendron mucronatum G. Don grown in subtropical Taiwan does not manifest endodormancy/ F. H. SungY. S. Chang
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Genetic diversity and relationship among peach cultivars based on Random Amplified Microsatellite Polymorphism (RAMP)/ Hsiu-Yu ChengWei-Chen YangJu-Ying Hsiao
Ultrastructural study and lipid formation of Isochrysis sp. CCMP1324/ Ching-Piao LiuLiang-Ping Lin
Taxonomic revision and cladistic analysis of Diphyscium (Family Diphysciaceae) of Taiwan/ Tzen-Yuh ChiangShang-Hsiung Lin
Three more new species of Ischaemum L. (Poaceae) from Kerala, India/ N. aviN. MohananT. ShajuM. S. Kiran RajR. Rajesh
 
   
 
   

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