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1
題名:Genotyping and assessment of genetic relationships in elite polycross breeding cultivars of sweet potato in Taiwan based on SAMPL polymorphisms     (14點)
以SAMPL多型性對台灣多向雜交甘諸品種之基因組分型與遺傳關係之解析
著者:Yu-Tien Tseng(曾俞槙) ;Hsiao-Feng Lo(羅筱鳳) ;Shih-Ying Hwang(黃士穎)
出版地區:台灣
出版城市:台北市
學科:生命科學
關鍵字:遺傳關係 ; 多向雜交 ; 微衛星多型性基因座選擇性擴增分子標誌 ; 甘藷 ; Genetic relationships ; Polycross ; SAMPL ; Sweet potato
刊名:Botanical Bulletin of Academia Sinica
頁碼:99-105
語言:英語
摘要: 中文摘要PDF ; 英文摘要PDF

本研究以微衛星多型性基因座選擇性擴增分子標誌(SAMPL)分析22個台灣多向雜交育種使用之甘藷優良品種之遺傳關係。甘藷優良品種包括來自中國及日本之品種,也有台灣之本地種及經由雜交及多向雜交育種而得之品種。在12個SAMPL引子對中,有7個引子對可擴增出19個基因座,另外5個引子對所擴增之條待型式模糊不清,不過於進一步分析。擴增出的19個基因座總共含有55個對個基因。利用非加權駢對之算術平均數的方法及主座標分析,結果顯示台灣本地種與中國及日本品部有較遠之遺傳關係,此點可能與台灣本地種在經過多年的獨立演化及篩選有關,而很可能的台灣本地種是源自爪哇及婆羅州。再者,多向雜交育成之品種與台灣本地種有較近的遺傳關係,因此利用SAMPL分子標誌可有效的解析?藷多向雜交品種之遺傳關係。
Selective amplification of microsatellite polymorphic loci (SAMPL) markers was used to analyze the genetic relationships between 22 elite cultivars of sweet potato [Ipomoea batatas (L.) Lam.] used in polycross breeding in Taiwan. These elite cultivars included varieties introduced from China and Japan and landraces from Taiwan as well as cultivars derived either from hybrid or polycross breeding programs. Among 12 SAMPL primer pairs tested, 7 amplified 19 loci while the other 5 SAMPL primer pairs gave ambiguous banding profiles. Fifty-five alleles were amplified in total. The unweighted pair group method with arithmetic mean (UPGMA) and principal coordinate analysis (PCA) of SAMPL data suggest that Taiwan landraces are distantly related to Chinese and Japanese cultivars after many years of independent selection and may have originated from Java and Brahman. Polycross derived cultivars are closely related to different landraces. Employment of SAMPL markers is efficient compared to other molecular marker methods, such as random amplified polymorphic DNA (RAPD) and simple sequence repeat (SSR), in assessing the genetic relationships of sweet potato cultivars used in the polycross breeding program in Taiwan.


    

本卷期目次
Botanical Bulletin of Academia Sinica
Carbohydrate metabolism in rice during callus induction and shoot regeneration induced by osmotic stress/ Wen-Lii HuangLi-Fei Liu
A novel bound form of plant invertase in rice suspension cells/ Cheng-Chung HsiaoRu-Huei FuHsien-Yi Sung
Conservation of Anoectochilus formosanus Hayata by artificial cross-pollination and in vitro culture of seeds/ Yih-Juh ShiauUei-Chin ChenShu-Ru YangHsin-Sheng Tsay
A morphometric analysis of infraspecific taxa within the Ixeris chinensis complex (Asteraceae, Lactuceae)/ Sung Soo WhangKyung ChoiRobert S. HillJae-Hong Pak
The efficiencies of various embryo rescue methods in interspecific crosses of Lilium/ Hai-Shan Chi
Molecular evidence for the sister relationship of the eastern Asia-North American intercontinental species pair in the Podophyllum group (Berberidaceae)/ Jian-Quan LiuZhi-Duan ChenAn-Ming Lu
Senecio kuanshanensis (Asteraceae), a new species from southern Taiwan/ Shih-Wen ChungChing-I Peng
A taxonomic and karyological study of the Codium geppiorum complex (Chlorophyta) in southern Taiwan, including the description of Codium nanwanense sp. nov./ Jui-Sheng ChangChang-Feng DaiJeng Chang
An assessment of DNA polymorphisms and genetic relationships of Casuarina equisetifolia using RAPD markers/ Kuen-Yih HoChern-Hsiung OuJenq-Chuan YangJu-Ying Hsiao
Genotyping and assessment of genetic relationships in elite polycross breeding cultivars of sweet potato in Taiwan based on SAMPL polymorphisms/ Yu-Tien TsengHsiao-Feng LoShih-Ying Hwang
 
   
 
   

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