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1
題名:Universal Primers for Amplification of the Complete Mitochondrial 125 rRNA Gene in Vertebrates    
萬用引子用以增幅脊椎動物完整粒線體12S rRNA基因
著者:Hurng-Yi Wang(王弘毅) ;Mung-Pei Tsai(蔡孟蓓) ;Ming-Chung Tu(杜銘章) ;Sin-Che Lee(李信徹)
出版地區:台灣
出版城市:台北市
學科:生命科學 ; 醫學綜合
關鍵字:12SL和12SR ; 引子 ; 聚合?鏈反應增幅作用 ; 脊椎動物 ; 12SR and 12SL ; Primers ; PCR amplification ; Vertebrates
刊名:Zoological Studies 
頁碼:61-66
語言:英語
摘要: 中文摘要PDF ; 英文摘要PDF

脊椎動物粒線體tRNAPHE和16S rRNA之保守區經比對後設計成引子12SR和12SL。這些萬用引子可以廣泛地使用於脊椎動物各個主群的動物種,而能利用聚合?鏈反應來增幅約1.3 kb的去氧核醣核酸片段。此產物的長度差異在各群脊椎動物間很小。進一步的序列分析顯示此片段含有完整的12S rRNA和tRNAPHE及約200 kb的16S rRNA。研究分類群後發現上述的引子能用以增幅完整粒線體12S rRNA。在12S rRNA基因被廣泛地用於不同層級類緣分析的同時,這些引子組更能被用在脊椎動物較高階的類緣關係。
The conserved regions of tRNAPHE and 16S rRNA in the vertebrate mitochondrial genome were compared in order to design the primers, 12SR and 12SL. These universal primers can be broadly used to amplify a 1.3-kb DNA fragment by polymerase chain reaction (PCR) over a wide range of major vertebrate lineages represented by the species listed in the text. There is little length variation of the PCR product among different taxa. Further sequence analysis revealed that the fragment contains complete lengths of 12S rRNA and tRNAVAL, and that the length of 16S rRNA is 200 bp. In tests through all representative taxa investigated, the above 2 primers could amplify the complete 12S rRNA gene from all representative taxa investigated. As the 12S rRNA gene is widely used for phylogenetic analyses among different hierarchies, the use of these primer sets for study of higher-category phylogenies in vertebrates now becomes possible.


    

本卷期目次
Zoological Studies 
Mitochondrial DNA Mutation and Depletion Are Associated with Decline of Fertility and Motility of Human Sperm/ Yau-Huei WeiShu-Huei Kao
Redescription of the Types of Ischikauia macrolepis Regan, 1908, an Extinct Cyprinid (Teleostei: Cyprinidae) from Taiwan and the Replacement in the Genus, Rasborinus Oshima, 1920/ I-Shiung ChenLee-Shing Fang
Xyrichtys trivittatus, a New Species of Razorfish (Perciformes: Labridae) from Hong Kong and Taiwan/ John E. RandallAndrew S. Cornish
Amida Lewis, with Description of a New Species (Coleoptera: Coccinellidae) from China/ Guo-Yue Yu
Cytochemical Profiles and Quantitative Analysis of Fiber Types in Trunk Muscle of Tigerperch, Terapon jarbua/ Shueh-Fen ChenBao-Quey Huang
Phylogeographic Variation in Mitochondrial DNA of Formosan White-bellied Rat Niviventer culturatus/ Fu-Hsiung HsuFei-Jann LinYao-Sung Lin
Burrow Architecture of the Spionid Polychaete Polydora villosa in the Corals Montipora and Porites/ Pi-Jen LiuHwey-Lian Hsieh
Sex Differences in the Responses of Serum Calcium Concentrations to Temperature and Estrogen in Tilapia, Oreochromis mossambicus/ Ching-Lin TsaiLi-Hsueh Wang
Universal Primers for Amplification of the Complete Mitochondrial 125 rRNA Gene in Vertebrates/ Hurng-Yi WangMung-Pei TsaiMing-Chung TuSin-Che Lee
Abundance and Movement of a Riparian Frog (Rana swinhoana) in a Subtropical Forest of Guandau Stream, Taiwan/ Yeong-Choy KamTe-Chih Chen
 
   
 
   

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