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項次 書目
1
題名:Participation of Glycoproteins on Zooxanthellal Cell Walls in the Establishment of a Symbiotic Relationship with the Sea Anemone, Aiptasia pulchella    
共生藻細胞壁醣蛋白參與海葵和其共生藻共生機轉的建立
著者:Ku-Lin Lin(林谷霖) ;Jih-Terng Wang(王志騰) ;Lee-Shing Fang(方力行)
出版地區:台灣
出版城市:台北市
學科:生命科學 ; 醫學綜合
關鍵字:共生藻 ; 刺絲胞動物 ; 胞內共生 ; 植物凝集素 ; 識別機制 ; Symbiodinium ; Cnidarians ; Endosymbiosis ; Lectin ; Recognition
刊名:Zoological Studies 
頁碼:172-178
語言:英語
摘要: 中文摘要PDF ; 英文摘要PDF

一般認為細胞膜上的醣蛋白(glycoprotein)是細胞間互相辨識的分子,本研究則進一步研究共生藻細胞壁上的醣蛋白與海葵宿主形成共生時所扮演的角色。當單離之共生藻受trypsin、α-amylase、N-glycosidase F或O-glycosidase處理後,其進入白化海葵細胞中,並恢復共生的能力明顯低於未處理之共生藻,若以lectin來遮蔽共生藻表面的醣蛋白時,亦有相同之效果,進一步以SDS-PAGE及西式點墨法分析共生藻表面之醣蛋白時,發現10條主要蛋白質染色帶中有5條是被鑑定為醣蛋白,其中醣基之結合模式,初步鑑定出有二種,即mannose-mannose和galactose-β(1-4)-N-acetyl glucosamine,而且發現量最多的醣蛋白(64 Kda)具有mannose的末端醣基,這些結果顯示,當共生藻與海葵(A. pulchella)形成共生關係時,共生藻表面之醣蛋白將扮演重要的角色。
Recent studies have revealed that glycoproteins on cell membranes are usually involved in the process of cell recognition. This study provides evidence to demonstrate the role of cell surface glycoproteins of zooxanthellae in establishing a symbiotic relationship between algae and a sea anemone host. When freshly isolated zooxanthellae from the sea anemone, Aiptasia pulchella, were incubated with trypsin, α-amylase, N-glycosidase F, or O-glycosidase, the algae could not infect bleached animals as efficiently as did the control algae. If the glycoproteins on the surface of the zooxanthellae were masked with lectins, the infection rate also declined significantly. Further, the glycoproteins on the cell walls of zooxanthellae were isolated and characterized with SOS-polyacrylamide gel electrophoresis and Western blotting. There are 10 protein bands in total revealed by Coomassie blue and silver staining, of which 5 are identified as glycoproteins. Two types of terminal sugar residues, mannose-mannose and galactose-β (1-4)-N-acetylglucosamine, were characterized among them. The most abundant glycoprotein with a molecular weight of 64 kDa carries a terminal sugar mannose. These data indicate that the glycoproteins on cell walls of zooxanthellae are a crucial factor in the successful establishment of the symbiotic relationship between zooxanthellae and A. pulchella.


    

本卷期目次
Zoological Studies 
Stress and Deviant Reproduction in Echinoderms/ John M. LawrenceJoan Herrera
Participation of Glycoproteins on Zooxanthellal Cell Walls in the Establishment of a Symbiotic Relationship with the Sea Anemone, Aiptasia pulchella/ Ku-Lin LinJih-Terng WangLee-Shing Fang
A Taxonomic Reappraisal of Montipora digitata Based on Genetic and Morphometric Evidence/ Ben Stobart
Community Structure and Functional Organization of Aquatic Insects in an Agricultural Mountain Stream of Taiwan: 1985-1986 and 1995-1996/ Sen-Her ShiehPing-Shih Yang
Polydora (Polychaeta: Spionidae) Species from Taiwan/ Hwey-Lian Hsieh
Pseudopolydora (Polychaeta: Spionidae) Species from Taiwan/ Hwey-Lian Hsieh
Argyrodes fissifrons Inhabiting Webs of Cyrtophora Hosts: Prey Size Distribution and Population Characteristics/ I-Min TsoLucia Liu Severinghaus
Allelochemical Induction of Hormone-metabolizing Microsomal Monooxygenases in the Fall Armyworm/ Simon J. Yu
The Irregular Sea Urchins (Echinodermata: Echinoidea) from Taiwan, with Descriptions of Six New Records/ Shyh-Min Chao
Resource Limitation Underlying Reproductive Strategies of Coral Reef Fishes: A Hypothesis/ Rong-Quen Jan
New Records of Sea Stars (Asteroidea: Echinodermata) from the Continental Shelf of Taiwan/ Shyh-Min Chao
 
   
 
   

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