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1
題名:Purification and Characterization of Black Porgy Muscle Cu/Zn Superoxide Dismutase    
黑鯛銅鋅型超氧歧化?之純化及其性質之研究
著者:Chi-Tsai Lin(林棋財) ;Tung-Liang Lee(李棟樑) ;Kow-Jen Duan(段國仁) ;Jong-Ching Su(蘇仲卿)
出版地區:台灣
出版城市:台北市
學科:生命科學 ; 醫學綜合
關鍵字:黑鯛 ; 銅鋅型超氧歧化? ; 熱穩定性 ; Black porgy ; Acanthopagrus schlegeli ; Cu/Zn superoxide dismutase ; Thermal stability
刊名:Zoological Studies 
頁碼:84-90
語言:英語
摘要: 中文摘要PDF ; 英文摘要PDF

生物體內超氧歧化?可當作對環境污染程度之生物指標,顯見其重要性。以黑鯛為材料,經測定其銅鋅型超氧歧化?存在六種組織中,但心臟、肝臟及雄性生殖器同時亦含猛型超氧歧化?。以其肌肉為材料,其粗抽取液經加熱及兩種管柱層析,可得到均質之純酵素。測得原態分子為33 kDa,次單元體為15.85 kDa,N端之胺酸為VLKAVCVLKGAGQTTGVV,比活性每毫克含3318單位,此?對pH、蛋白質水解?及80℃加熱處理均甚穩定。
Superoxide dismutase (SOD) has been proposed to be used as a bioindicator for environmental impact assessment. From a survey of SOD activity in black porgy, Acanthopagrus schlegeli, we found that Cu/Zn SOD was distributed rather evenly in 6 tissues, and in addition, only heart, liver, and testis had Mn SOD. We purified Cu/Zn SOD from muscle to homogeneity by a procedure that includes heating at 65℃ and fractionation on 2 chromatographic columns. The molecular mass of the native enzyme was 33 kDa and that of the subunit mass, deduced from a cDNA sequence, was 15.85 kDa. Thus the native enzyme appeared to be a homodimer. It had an N-terminal sequence of VLKAVCVLKGAGQTTGVV. The specific activity was 3318 u/mg. The enzyme had a broad optimum pH range of 5.8 to 11.2 and was resistant both to proteolysis by trypsin and chymotrypsin and to heat denaturation. The thermal inactivation rate constant of the enzyme at 80 ℃ was -0.0237 min-1 and the half life for inactivation was 27.8 min.


    

本卷期目次
Zoological Studies 
A Review of Lanternfishes (Families: Myctophidae and Neoscopelidae) and Their Distributions around Taiwan and the Tungsha Islands with Notes on Seventeen New Records/ John Ta-Ming WangChe-Tsung Chen
Three New Coelotes Spiders (Araneae: Amaurobiidae) from Taiwan/ Xin-Ping WangI-Min TsoHai-Yin Wu
The Nominal Invalidity of the Cyprinid Genus, Parasinilabeo, with Descriptions of a New Genus and Species/ Rui-Feng SuJun-Xing YangGui-Hua Cui
Schismatogobius fuligimentus, a New Species of Freshwater Goby (Teleostei: Gobiidae) from New Caledonia/ I-Shiung ChenKwang-Tsao Shao
Phylogeny of the Specialized Schizothoracine Fishes (Teleostei: Cypriniformes: Cyprinidae)/ Zi-Ming ChenYi-Feng Chen
Clarias microstomus, a New Species of Clariid Catfish from Eastern Borneo (Teleostei: Siluriformes)/ Heok-Hee Ng
Seasonal Activity and Reproduction of Two Syntopic White-Toothed Shrews (Crocidura attenuata and C. kurodai) from a Subtropical Montane Forest in Central Taiwan/ Hon-Tsen YuTing-Wen ChengWen-Hao Chou
Naso reticulatus, a New Unicornfish (Perciformes: Acanthuridae) from Taiwan and Indonesia, with a Key to the Species of Naso/ John E. Randall
Agrostaleyrodes arcanus, a New Genus and Species of Whitefly (Homoptera: Aleyrodidae) from Taiwan/ Chiun-Cheng KoLiang-Yih ChouWen-Jer Wu
A New Synaphobranchid Eel, Dysomma longirostrum (Anguilliformes: Synaphobranchidae), from the Northeastern Coast of Taiwan/ Yu-Yun ChenHin-Kiu Mok
Purification and Characterization of Black Porgy Muscle Cu/Zn Superoxide Dismutase/ Chi-Tsai LinTung-Liang LeeKow-Jen DuanJong-Ching Su
Phylogeographic Structure of the Formosan Wood Mouse, Apodemus semotus Thomas/ Fu-Hsiung HsuFei-Jann LinYao-Sung Lin
 
   
 
   

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