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1
題名:Variation in Mitochondrial DNA and Phylogenetic Relationships of Fishes of the Subfamily Sparinae (Perciformes: Sparidae) in the Coastal Waters of Taiwan    
臺灣沿岸海域鯛亞科魚類粒線體DNA核?酸序列之變異及種間親緣關係
著者:Chuen-Tan Jean(簡春潭) ;Cho-Fat Hui(許祖法) ;Sin-Che Lee(李信徹) ;Che-Tsung Chen(陳哲聰)
出版地區:台灣
出版城市:台北市
學科:生命科學 ; 醫學綜合
關鍵字:粒線體 ; 序列變異 ; 種間關係 ; 鯛亞科 ; mtDNA ; Sequence variation ; Interspecific relationships ; Sparinae
刊名:Zoological Studies 
頁碼:270-280
語言:英語
摘要: 中文摘要PDF ; 英文摘要PDF

本研究比較分析澳洲黑鯛(Acanthopagrus australis)、灰鰭鯛(A. berda)、黃鰭鯛(A. latus)、黑鯛(A. schlegeli)及黃錫鯛(Sparus sarba)各12尾標本粒線體DNA之D-loop區、tRNAPhe基因及部兮12S rRNA基因之核?酸序列,以探討其核?酸序列之變異及闡明其種間親緣關係。 種內及種間核?酸序列之排序、比較結果,D-loop區、tRNAPhe基因及部分12S rRNA基因核?酸序列之長度,在澳洲黑鯛分別?932-948 bp、71 bp及401 bp;在灰鰭鯛?931-938 bp、71 bp及400 bp;在黃鰭鯛?942-1003 bp、71 bp及401 bp;在黑鯛?940 bp、71 bp及406 bp;在黃鰭鯛?971-973 bp、71 bp及398 bp。tRNAPhe基因及12S rRNA基因相當保守,變異很小,D-loop區之左、右區段含有較高比例之腺嘌呤(A, adenine)及較低比例之鳥糞嘌呤(G, guanine),且具有較大之序列及長度變異;而D-loop區之中央區段則含有較低比例之腺嘌呤及較高比例之鳥糞嘌呤,且較左、右區段保守,變異較小。出現在其它脊椎動物D-loop區之保守序列(conserved sequence elements) TAS、CSB-2及CSB-3亦出現在鯛亞科魚類之D-loop區。 種內兩兩標本間之Tamura-Nei序列距離,澳洲黑鯛?0.0021-0.0130,灰鰭鯛?0.0000-0.0115,黃鰭鯛?0.0014-0.0209,黑鯛?0.0014-0.0093,黃鰭鯛?0.0000-0.0056;而種間之Tamura-Nei序列距離在黑鯛屬內?0.1498-0.1914、在黑鯛屬與鯛屬間則?0.2386-0.2708。依據種間序列距離使用UPGMA聚類方去繪出之系統樹顯示,鯛亞科五種魚明顯分成二群,一群包括澳洲黑鯛、灰鰭鯛、黃鰭鯛及黑鯛,即黑鯛屬;另一群僅有黃鰭鯛,即鯛屬。而黑鯛屬四種魚之親緣關係則澳洲黑鯛與黑鯛最接近,其次依序?灰鰭鯛、黃鰭鯛。此系統樹與使用同功異構?電泳法所獲者一致,但與形態測定方法所獲者不一致。
Mitochondrial DNA (mtDNA) sequences that included the non-coding D-loop region, the tRNAPhe gene, and a part of the 12S rRNA gene from 60 individuals belonging to 5 Sparinae species were sequenced to document the mtDNA sequence variations as well as to elucidate the phylogenetic relationships of these species. The intraspecific and interspecific comparisons of aligned sequences demonstrate that the tRNAPhe gene and the 12S rRNA gene are highly conserved. The left and right domains of the D-loop region contain a higher A content and a lower G content, and are highly variable in both sequences and lengths. The central domain of the D-loop region contains a lower A content and a higher G content, and is more conserved than the other two domains. The conserved sequence elements TAS, CSB-2, and CSB-3, which have been reported previously from other vertebrates, are maintained in Sparinid fishes. The intraspecific pairwise sequence distances using the Tamura-Nei model ranged between 0.0021-0.0130 in Acanthopagrus australis, 0.0000-0.0115 in A. berda, 0.0014-0.0209 in A. latus, 0.0014-0.0093 in A. schlegeli, and 0.0000-0.0056 in Sparus sarba. The interspecific pairwise sequence distances using the Tamura-Nei model, ranged from 0.1498 to 0.1914 between species of Acanthopagrus, and from 0.2386 to 0.2708 between species of the genera Acanthopagrus and Sparus. The phylogenetic tree constructed by UPGMA based on the mtDNA sequence data shows the same topology as that based on allozyme electrophoresis data, but it is different from that based on morphometric data.


    

本卷期目次
Zoological Studies 
Transgenic Fish: Ideal Models for Basic Research and Biotechnological Applications/ Thomas T. ChenJenn-Kan LuClara M. ChengChun-Mean Lin
Comparison of the Effects of Copper on Respiration and Its Accumulation in Tissue in the Hard Clam Meretrix lusaria/ I-Ming Chen
New Records of Calcinus Hermit Crabs (Decapoda: Anomura: Diogenidae) from Taiwan/ Hsi-Te ShihHsiang-Ping Yu
The Development of the Catecholaminergic Nervous System in Starfish and Sea Cucumber Larvae/ Chang-Po ChenChin-Hsiu TsengBih-Yuh Chen
Feed Administration of Estradiol-17s Stimulates Female Differentiation in Juvenile Grey Mullet Mugil cephalus/ Ching-Fong ChangShih-Chien LanBonnie Sun Pan
The Effects of Eyestalk Ablation on Oxygen Consumption and Ammonia-N Excretion of Juvenile Shrimp Penaeus monodon/ Fan-Hua NanShyn-Shin SheenYou-Tsu ChengShiu-Nan Chen
Variation in Mitochondrial DNA and Phylogenetic Relationships of Fishes of the Subfamily Sparinae (Perciformes: Sparidae) in the Coastal Waters of Taiwan/ Chuen-Tan JeanCho-Fat HuiSin-Che LeeChe-Tsung Chen
Heat Shock Protein Expressed by the Adult Fluke, Clonorchis sinensis in Vitro/ Jyh-Wei ShinChing-Yu ChenShiu-Nan Chen
New Records of the Commensal Gobiid Genus, Bryaninops (Pisces: Gobiidae), from Southern Taiwan/ I-Shiung ChenChiao-Chuan HanLee-Shing Fang
 
   
 
   

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