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項次 書目
1
題名:Construction of an Indian Muntjac BAC Library and Production of the Most Highly Dense FISH Map of the Species    
著者:Chyi-Chyang LinPei-Ching HsuTzai-Shiuan LiShu-Ju LiaoYa-Ming ChengLie-Jiau HsiehYueh-Chun Li
出版地區:台灣
出版城市:台北市
學科:生命科學 ; 醫學綜合
關鍵字:BAC library ; FISH mapping
刊名:Zoological Studies
頁碼:282-292
語言:英語
摘要: 英文摘要PDF

Following completion of the genome sequences of some mammalian species, comparative genomic studies in mammals have been actively conducted to assess gene changes or to identify syntenic conservation during evolution. The Indian muntjac (Muntiacus muntjak vaginalis) (2n=6 in the female and 7 in the male) may have evolved from an ancient deer species with a karyotype 2n=70 through extensive chromosome rearrangements creating the lowest chromosome number of a mammalian species. Therefore, the species has become a good resource for studying syntenic conservation among deer species. An Indian muntjac bacterial artificial chromosome (BAC) library that contains 126,336 individual BAC clones with an average insert size of 80 kilobases was obtained in this study. The frequency of clones with inserts was 88%, and thus this library corresponds to approximately 4x coverage of the Indian muntjac genome. Individual chromosomal locations of 1619 BAC clones on the Indian muntjac metaphase chromosomes were identified by fluorescence in situ hybridization (FISH). Among these clones, 1517 BAC clones were mapped onto specific loci, and 102 BAC clones were mapped onto the centromeric region. This provides the most highly dense FISH BAC clone map for the species. This densely ordered map can be used as a blueprint for comparative FISH mapping studies of other deer species in order to investigate the mechanism of genomic rearrangement and karyotypic evolution. Moreover, centromeric BAC clones will provide an excellent resource for studying the structure and function of mammalian centromeres.


    

本卷期目次
Zoological Studies
Effects of Troop Size on Social Relations among Male Formosan Macaques, Macaca cyclopis/ Tai-Jung LinGovindasamy AgoramoorthyChih-Chien HuangMinna J. Hsu
Description of Adults, Preimaginal Phases, and the Venom Apparatus of a New Species of Aspilota Forster from Spain (Hymenoptera: Braconidae)/ Maximilian FischerJosep TormosXavier PardoJosep Daniel Asis
Spatial and Seasonal Variations in Chaetognath Assemblages in Two Subtropical Marine Inlets with Different Hydrographical Characteristics/ Pan TseSami SouissiJiang-Shiou HwangQing-Chao ChenChong Kim Wong
Factors Affecting Invasive Species Abundance: The Barbary Ground Squirrel on Fuerteventura Island, Spain/ Marta Lopez-DariasJorge M. Lobo
Construction of an Indian Muntjac BAC Library and Production of the Most Highly Dense FISH Map of the Species/ Chyi-Chyang LinPei-Ching HsuTzai-Shiuan LiShu-Ju LiaoYa-Ming ChengLie-Jiau HsiehYueh-Chun Li
Cooption of Neo-X and Neo-Y Chromosomes in Drosophila albomicans/ Sung-Han LinYu-Yang HuangHwei-Yu Chang
Development of Ovarian Tissue and Female Germ Cells in the Protandrous Black Porgy, Acanthopagrus schlegeli (Perciformes, Sparidae)/ Mong-Fong LeeJing-Duan HuangChing-Fong Chang
A Sea Anemone Outbreak Eliminates Damselfish Territories from Fringing Reefs in Southern Taiwan/ Tsen-Chien ChenCheng-Tze HoRong-Quen Jan
Sexual Polymorphism in a Population of Strombus canarium Linnaeus, 1758 (Mollusca: Gastropoda) at Merambong Shoal, Malaysia/ Zaidi Che CobAziz ArshadMohd Hanafi IdrisJapar Sidik BujangMazlan Abd Ghaffar
Apocyclops ramkhamhaengi sp. nov. (Copepoda: Cyclopoida) in a Culture Originating from Brackish Waters of Chang Island, Trat Province, Thailand/ Supawadee ChullasornPawana KangtiaKhwanruan PinkaewFrank D. Ferrari
Multi-Gene Analyses of the Phylogenetic Relationships among the Mollusca, Annelida, and Arthropoda/ Donald J. ColganPatricia A. HutchingsEmma Beacham
Differences in Morphological Traits between Two Sibling Species, Drosophila ananassae and D. pallidosa/ Chavali VishalakshiBashisth Narayan Singh
Mitochondrial DNA Diversity of Tor tambroides Valenciennes (Cyprinidae) from Five Natural Populations in Malaysia/ Yuzine B. EsaSiti Shapor SirajSiti Khalijah DaudKhairul Adha A. RahimJeffrine Rovie Ryan JapningSoon Guan Tan
Molecular Phylogenetics among Three Families of Bats (Chiroptera: Rhinolophidae, Hipposideridae, and Vespertilionidae) Based on Partial Sequences of the Mitochondrial 12S and 16S rRNA Genes/ Xiao-Ming GuShu-Yan HeLei Ao
 
   
 
   

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