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題名:CLONING AND CHARACTERIZATION OF A COPPER/ZINC-SUPEROXIDE DISMUTASE GENE FROM ASPERGILLUS JAPONICUS    
麴菌銅鋅型超氧歧化酶基因選殖
著者:Chi-Tsai Lin(林棋財) ;Ming-Tse Lin(林銘澤) ;Dey-Chyi SHEU(許垤棊) ;Kow-Jen Duan(段國仁)
出版地區:台灣
出版城市:台北市
學科:生命科學
刊名:Taiwania
卷期:39卷1、2期合刊(1994.10)
頁碼:73-79
語言:英語
摘要: 中文摘要PDF ; 英文摘要PDF

依據玉米Cu/Zn-SOD之核酸序列,合成兩股引子,並以麵藺cDNA當模板,以PCR 方法增生出一段0.3 kb DNA' 經次選殖及定序,其序列與玉米Cu/Zn-SOD之序列同 殖性高達73% '進一步以此0.3 kb DNA當探針,進行翹菌基因庫之篩選,結果篩得 一株含有0.4 kb之正反應株,經定序分析,發現可推導出含120倍胺基酸序列,其 序列和其它生物Cu/Zn-SOD 之序列問質性很高,並含有與鋼和錚進行相對應 Ccoordinating) 之胺基酸序列。
In order to clone a Cu/Zn-SOD gene from Aspergillus japonicus, the highly 、conserved regions of the SOD sequence of maize and other species were comp訂ed and two oligonucleotides were synthesized. The Aspergillus japonicus cDNA was then used as the template to get a 0.3 kb fragment by PCR technique. The 0.3 kb DNA fragment has high homology compared with maize SOD-4 cDNA. Then we used the 0.3 kb fragment as prob巴to screen Aspergillus japonicus genomic Iibraries. A 0.4 kb DNA fragment from purified positive plaque was subcloned into pGEM-7zf(+) vector and sequenced. Nucleotide sequence analysis of this c10ne revealed that it comprised an open reading frame coding for 120 amino acid residues. The encoded polypeptide is highly homologous with other plant SODs. The residues required för coordinating copper and zinc are also conserved as they are among all reported Cu/Zn-SOD sequences.


    

本卷期目次
Taiwania 39卷1、2期合刊 (1994.10)
THE FLORA OF TAIPINGTAO (ABA ITU ISLAND)/ Tseng-chieng HuangShing-Fan HuangKuo-Cheng Yang
THE FLORA OF TUNGSHATAO (PRATAS ISLAND)/ Tseng-chieng HuangShing-Fan HuangTsung-Hsin Hsieh
NOTES ON THE FLORA OF TAIWAN (17)--Elatine L. (ELATINACEAE)/ Tseng-chieng Huang
CRETACEOUS PALYNOMORPH OF TAIWAN (1)--Huangasporites genus nov/ Cheng-Long Shaw
NOTES ON THE FLORA OF TAIWAN(18)--Tribulus L. (Zygophyllaceae )/ Tseng-chieng HuangTsung-Hsin Hsieh
CLONING AND CHARACTERIZATION OF A COPPER/ZINC-SUPEROXIDE DISMUTASE GENE FROM ASPERGILLUS JAPONICUS/ Chi-Tsai LinMing-Tse LinDey-Chyi SHEUKow-Jen Duan
 
   
 
   

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