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1
題名:Biochemical Properties of Sweet Potato Rnases    
甘薯核醣核酸水解酵素生化性質的探討與分析
著者:Yu-Ligh Liou(劉禹利) ;You-Di Liao(廖有地) ;Yih-Ming Chen(陳益明) ;Chu-Yung Lin(林秋榮) ;Kai-Wun Yeh(葉開溫)
出版地區:台灣
出版城市:台北市
學科:生命科學
刊名:Taiwania
卷期:40卷2期(1995.6)
頁碼:183-191
語言:英語
摘要: 中文摘要PDF ; 英文摘要PDF

我們利用核醋核酸電泳膠片技術已經成功地分析出甘薯(台農57 號)核醋核酸 水解酵素的一些蛋白質特性及基本性質。在甘薯的根、莖、葉中有介於14-47 kDa分 子量的五群核醋核酸水解酵素(A-E) 恆定表現。然而卻有一低分子量的守9 , 000 kDa) 核醋核酸水解酵素在甘薯老化的葉片裡大量合成並且表現出來,我們稱此特 殊表現的蛋白質為F 群核醋核酸水解酵素。在此篇文章中,我們探討這六群核醋核 酸水解酵素之生化活性與pH 值、離子種類和濃度的關聯性,並利用SDS 和2- mercaptoethanol 變性劑觀察此類酵素的生化活性。
Six groups of ribonucleases (RNases) activities from Sweet potato (/pomoea batatas Lam. Cv. Tainong 57) leaves were identified and characterized by an RNA-cast polyacrylamide gel. Among these ribonucleases, five were major isoforms, and were designated to A-E groups, ranged in size from 14 to 47 kilodalton. They were consistentIy expressed in root, stem and leaf. In addition, a novel RNase isoform,' designated F group, with a molecular mass about 6000 dalton, expressed specifically in the aging leaf. In this paper the biochemical properties of six groups RNase isoforms were characterized with catalytic ions, pH v alu巴, denaturing reagent of SDS and 2-mercaptoethanol.


    

本卷期目次
Taiwania 40卷2期 (1995.6)
A Newly Recorded Pediastrum Species in Taiwan/ Jiunn-Tzong WuChyong-Ere Hsieh
Three New Records of Thermophilic Fungi from Taiwan/ Kuei-Yu ChenZuei-Ching Chen
The Formation of Lenticels on the Branches of Ficus microcarpa L. f./ Ling-Long Kuo-HuangLi-Fen Hung
Notes on Some Foliicolous Lichenized Fungi from Taiwan/ Chao-Hsuan Chung
Notes on the Flora of Taiwan (22)--The Genus Ajuga L. (Lamiaceae )/ Tsung-Hsin HsiehTseng-chieng Huang
Plant in Response to Copper Toxicity/ Ching-Chien TsayLi-Wen WangYung-Reui Chen
Biochemical Properties of Sweet Potato Rnases/ Yu-Ligh LiouYou-Di LiaoYih-Ming ChenChu-Yung LinKai-Wun Yeh
Notes on the Flora of Taiwan (21)--The Genus Asarum L. (Aristolochiaceae)/ Shing-Fan HuangTsung-Hsin HsiehTseng-chieng Huang
 
   
 
   

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