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項次 書目
1
題名:A Step-by-Step Optimization Guide for Applying Tissue Specific RNA in-situ Hybridization to Non-Model Plant Species    
在非模式植物發育研究中應用基因表現原位雜交技術的實驗技巧研究
著者:Chun-Neng WangYung-Chan Chang(張詠嬋) ;Chun-Hsien Wu(吳俊賢)
出版地區:台灣
出版城市:台北市
學科:生命科學
刊名:Taiwania
卷期:53卷4期(2008.12)
頁碼:383-393
語言:英語
摘要: 中文摘要PDF ; 英文摘要PDF

基因表現1單位雜交技術是結合了傳統的植物切片及當代的分子基因表現分析的新 興植物學技術'近年來已成為研究植物基因分子發育學者所必備的技術。然而由於其實 驗步驟繁雜,許多步轉必須注意RNA 酵素污染、探針的精確性、探針偵測信號的強弱 及組織埋蠟切丹和酵素前處理的過程,常使許多實驗室望而卻步此技術。本文廣泛的探 討了如何一步步應用原位雜交技術至非模式檻物物種的詳細步騁,並實際提供我們成功 發展技衛的數據,文末並特別討論本技術最重要的幾倍實驗步驛疑難處理原則,希望能 幫助新近此領域的研究者一個方向指南。
Tissue specific RNA in-situ hybridization is a critical technique required for botanists to examine exact gene expression domain within plant tissues. This powerful technique provides a way to examine the spatial and temporal pa眨巴rns of gene expression of plants and animals in developmental stages content and at tissue cellular level. However, this technique has been largely hampered by difficulties of obtaining reliable protocols for non-model organism. Here we offer our lab experiences on how to optimize in-situ hybridization process among various plant species. This step-by-step guide however should not be regarded as the only solution to resolve the difficulties of RNA tissue specific RNA in-situ hybridization. Instead, the key optimization steps including tissue fixation time, proteinase/pronase treatment, probe length/content and hybridization time are priority issues to be tested first. We further summarized some recent advance of literature on alternative ways to conduct in-situ hybridization process.


    

本卷期目次
Taiwania 53卷4期 (2008.12)
Vegetation Pattern and Woody Species Composition of a Broad-Leaved Forest at the Upstream Basin of Nantzuhsienhsi in Mid-southern Taiwan/ Kuoh-Cheng YangChang-Fu HsiehLi-Hao KuanJyh-Feng SuShau-Ting Chiu
Current Status of the Systematics of Astragalus L. (Fabaceae) with Special Reference to the Himalayan Species in India/ Lal Babu ChaudharyTikam Singh RanaKumar Kamal Anand
Tree Diversity and Structure of Andaman Giant Evergreen Forests, India/ M. RajkumarN. Parthasarathy
Aeropalynological Study of Kinmen Island, Taiwan/ Shu-Yue HuangArthur HsiaoSu-Hwa CHEN
A Step-by-Step Optimization Guide for Applying Tissue Specific RNA in-situ Hybridization to Non-Model Plant Species/ Chun-Neng WangYung-Chan ChangChun-Hsien Wu
Various Microscopic Methods for Investigating the Venuloid Idioblasts of Pteris grevilleana Wall./ Tzu-Tong KaoShiang-Jiuun CHENWen-Liang ChiouYi-Chun ChuangLing-Long Kuo-Huang
A New Species of Hedychium (Zingiberaceae) from Southern Laos/ Pornpimon Wongsuwan
A New Species of Kaempferia (Zingiberaceae) from Southern Laos/ Chayan PicheansoonthonSupachai Koonterm
Silene fissipetala (Caryophyllaceae), the Correct Name for S. fortunei from Continental China and Taiwan/ J. F. Veldkamp
Two Species of Kernia (Plectomycetes) in Taiwan/ Jong-How ChangYei-Zeng Wang
Rivina humilis L. (Phytolaccaceae), a Newly Naturalized Plant in Taiwan/ Yen-Hsueh TsengChih-Chiang WangYun-Tsao Chen
 
   
 
   

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