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1
題名:Various Microscopic Methods for Investigating the Venuloid Idioblasts of Pteris grevilleana Wall.    
利用不同的顯微鏡技術研究翅柄鳳尾蕨(Pteris grevilleana Wall.)的脈狀異形細胞
著者:Tzu-Tong Kao(高資棟) ;Shiang-Jiuun CHEN(陳香君) ;Wen-Liang Chiou(邱文良) ;Yi-Chun Chuang(莊以君) ;Ling-Long Kuo-Huang(黃玲瓏)
出版地區:台灣
出版城市:台北市
學科:生命科學
刊名:Taiwania
卷期:53卷4期(2008.12)
頁碼:394-400
語言:英語
摘要: 中文摘要PDF ; 英文摘要PDF

脈狀異形細胞(venuloid idioblast) (亦稱為假脈)泛指外觀像是葉脈的構造。在鳳尾鼓 屬(Pteris) 中的脈狀異形細胞,是一種細胞壁特別厚的長形表皮細胞。在本研究中,我 們利用各種光學顯微鏡及掃描式電子顯微鏡技術研究翅柄鳳尾脹的脈狀異形細胞。利用 半偏光的光學顯微鏡技巧以及冷凍式桌上型掃描式電子顯微鏡技術'可以有效的觀察該 異形細胞的形態並且偵測其分布。該異形細胞的邊緣為波浪狀,兩端尖或園,分布在營 養葉及抱子葉的上下表皮,其可在脈上、脈間或葉緣被找到,但不會出現在羽軸、小羽 軸或者假抱膜上。就能量分散X 射線光譜儀及組織化學染色的結果,顯示該異形細胞具 有二氧化矽'而運用濕式灰化法(wet oxidation) 得知其形態即類似於該異形細胞的矽酸 體(silica body) 。因此,翅柄鳳尾肢的脈狀異形細胞可歸類為一種過去在鐵線鼓及書帶放 類群中所被發現的矽異形細胞(spicular cells) 。
Venuloid idioblasts are vein-like structures. In Pteris, they are long epidermal ceIls with very thick ceIl walIs. In this study, venuloid idioblasts of Pteris grevilleana were investigated with various light microscopic (LM) and scanning electron microscopic (SEM) techniques and the main pu巾oses ofthese microscopic techniques are summarized and discussed. To investigate the morphology and distribution of venuloid idioblasts, pa此ial polarization LM technique and cryo-tabletop-SEM technique were used. The idioblasts had lobed margins, acute or round ends, and could be found on both upper and lower epidermis of fertile fronds and st巴rile fronds. They are distributed on veins, interveinal regions, and leaf margins, but not on costul郎, costae, and false indusia. By using histochemical staining and SEM with energy dispersive X-ray spectrometer (EDS), it was confirmed that the idioblasts contained silicon. In addition, the venuloid-idioblast-like silica bodies were extracted by wet oxidation method. These indicate that the venuloid indioblast in P. grevilleana is a kind of spicular ceIl (Iong epidermal celIs containing silica bodies, which are found in Adiantoids and Vittarioids).


    

本卷期目次
Taiwania 53卷4期 (2008.12)
Vegetation Pattern and Woody Species Composition of a Broad-Leaved Forest at the Upstream Basin of Nantzuhsienhsi in Mid-southern Taiwan/ Kuoh-Cheng YangChang-Fu HsiehLi-Hao KuanJyh-Feng SuShau-Ting Chiu
Current Status of the Systematics of Astragalus L. (Fabaceae) with Special Reference to the Himalayan Species in India/ Lal Babu ChaudharyTikam Singh RanaKumar Kamal Anand
Tree Diversity and Structure of Andaman Giant Evergreen Forests, India/ M. RajkumarN. Parthasarathy
Aeropalynological Study of Kinmen Island, Taiwan/ Shu-Yue HuangArthur HsiaoSu-Hwa CHEN
A Step-by-Step Optimization Guide for Applying Tissue Specific RNA in-situ Hybridization to Non-Model Plant Species/ Chun-Neng WangYung-Chan ChangChun-Hsien Wu
Various Microscopic Methods for Investigating the Venuloid Idioblasts of Pteris grevilleana Wall./ Tzu-Tong KaoShiang-Jiuun CHENWen-Liang ChiouYi-Chun ChuangLing-Long Kuo-Huang
A New Species of Hedychium (Zingiberaceae) from Southern Laos/ Pornpimon Wongsuwan
A New Species of Kaempferia (Zingiberaceae) from Southern Laos/ Chayan PicheansoonthonSupachai Koonterm
Silene fissipetala (Caryophyllaceae), the Correct Name for S. fortunei from Continental China and Taiwan/ J. F. Veldkamp
Two Species of Kernia (Plectomycetes) in Taiwan/ Jong-How ChangYei-Zeng Wang
Rivina humilis L. (Phytolaccaceae), a Newly Naturalized Plant in Taiwan/ Yen-Hsueh TsengChih-Chiang WangYun-Tsao Chen
 
   
 
   

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