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1
題名:烏魚精液保存試驗-Ⅰ. 生物特性之探討及稀釋液效果之研究    
Experiment on Cryogenic Preservation of Grey Mullet Sperm -Ⅰ.Study on the Biological Characteristics and Effect of Protectants
著者:趙乃賢(Nai-Hsien Chao) ;陳惠彬(Huei-Pin Chen) ;曾雷強(Lei-Chiang Tseng) ;蘇祐明(You-Ming Su) ;廖一久(I-Chiu Liao)
出版地區:台灣
出版城市:基隆市
學科:農林漁牧礦科學
刊名:臺灣水產學會刊
卷期:2卷1期(1973.6)
頁碼:31-41
語言:繁體中文
摘要: 中文摘要PDF

1972年12月至1973年2月,繼去年度之鳥魚精液保存試驗,再度配合東港分所之烏魚人工大量繁殖試驗,進行烏魚精液保存方法之探討。本次,首先研討烏魚精液之生物特性,續作5℃低溫及-196℃超低溫保存,略有所得: (1)在產卵洄游期游至東港沿岸之烏魚雄魚之GSI約為4.17~19.65不等,精液之pH值為7.4,精子之本體是2.3μ×1.4μ大小之長圓體,每cc精液平均約5.3×10^10個精子,精子在普通海水中具有最佳活力,鹽分較高或較低的水反抑制其活力。 (2)精液保存於5℃時,純精液至少維持活力15天,添加稀釋液之濃度各以Glycerin(1:1)5%,DMSO(1:1)5%,Sodium citrate 0.5%及1%,Glucose 3%,5%及6%以及Lactose 12%者較佳,但保存日數較不加任何添加物之純精液者減短。 (3)至於保存於-196℃液態氮之精液均添加稀釋液,目前已試驗部分係以添加Glycerin 5%及DMSO 5%之組最好,保存48天仍具活力,唯因其他處理仍在保存中,尚待比較方能定論。 (4)此次授精情形最好者為添加Glycerin 5%,保存於-196℃中39天之精液,其受精率及孵化率分別為15.63%及61.33%。
The preliminary experiment on the cryogenic preservation of grey mullet (Mugil cephalus) sperm of 1971/1972 was continued during the period from December,1972 to February, 1973.Accompanied with the artificial propagation work at Tungkang Marine Laboratory, this experiment was carried out with the advantage to get semen and to have the opportunities of fertilization with nice eggs of good spawners. The first part is to study the biological characteristics of gonad, semen and sperm. The GSI of male mullet is ranged from 4.17 to 19.65 during the spawning season. The pH value of semen is 7.4.The main body of spermatozoa measures 2.3 μ×1.4 μ. There are about 5.3×10^10 sperms in each cc of semen. Sperms show the best motility as soon as the semen is mixed with drops of seawater but not with water of higher or lower salinity. The second part of this experiment is 5℃ preservation. Sperm motility was retained up to 15 days in the case of non-protectant and less in the case of adding protectant such as glycerin and dimethyl sulfoxide (DMSO) or nutrient such as glucose and lactose. The best result was obtained in the group of glycerin 7.5% preparation. After 7 days of preservation at 5℃, the fertilization rate was 0.13%. The hatching rates are not good in all the 5℃ preserved groups. The third part is -196℃ preservation in liquid nitrogen. The best results are in the groups of glycerin 5% and DMSO 5% preparation. Among the samples thawed in this experiment, the best motility was retained up to 48 days and the best fertilization rate is 15.63% with a hatching rate of 61.33% in the 39-day glycerin 5% preparation. Because a few samples are still preserved in liquid nitrogen (LN2), it is too soon to make a conclusion. Further study is absolutely needed for the purpose of 1ong-term cryogenic preservation of semen and study on the proper protectants, kind of diluents and their respective properconcentrations will be the main subjects.


    

本卷期目次
臺灣水產學會刊 2卷1期 (1973.6)
印度洋黃鰭鮪的年齡、成長和族群構造/ 黃哲崇孫寧楊榮宗
烏魚精液保存試驗-Ⅰ. 生物特性之探討及稀釋液效果之研究/ 趙乃賢陳惠彬曾雷強蘇祐明廖一久
魚類病原菌Aeromonas liquefaciens之研究-Ⅱ 病原性與毒素物質之關係/ 郭光雄
Aeromonas liquefaciens Isolated from a Skin Rot Disease of Formosa Snakehead, Channa Maculata (LACÉPÈDE), in Taiwan/ Yan-Ling SongHuu-Yun ChungGuang-Hsiung Kou
魚肉組織中氧化三甲胺含量之快速定量法/ 錘忠勇陳學良吳全耀黃紹國
臺灣漁船漁獲物放射性污染之研究/ 黃蔭樨黃耀文朱鐵吉
東海南區、臺灣海峽產白口魚形態測定值之比較/ 曾萬年劉錫江
基隆港捕獲魚具類之重金屬含量/ 鄭森雄黃耀文李義生王果行鄭淑楨
 
   
 
   

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