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1
題名:Differentiation of Four Eimeria Species Commonly Seen in Taiwanese Rabbits by RAPD    
臺灣常見兔艾美球蟲的RAPD分子鑑定
著者:Ming-Hsien Li(李明憲) ;Chao-Tsai Liao(廖朝財) ;Wei-Chih Ou(歐威志) ;Pei-Lain Chen(陳碧蓮) ;Chiung-Hua Huang(黃瓊華) ;Yu-Hsuan Chen(陳妤瑄) ;Hai-I Huang(黃海義) ;Hong-Kean Ooi(黃鴻堅)
出版地區:台灣
出版城市:新北市
學科:生命科學 ; 醫學綜合
關鍵字:隨機增幅多型性DNA ; 艾美球蟲 ; 鑑定 ; ; RAPD ; Eimeria ; Differentiation ; rabbit
刊名:Taiwan Veterinary Journal
卷期:38卷4期(2012.12)
頁碼:227-232
語言:英語
摘要: 中文摘要PDF ; 英文摘要PDF

以11個非特異性引子(G2, E111-E120)進行隨機增幅多型性DNA(RAPD)鑑別4種兔艾美球蟲(Eimeria piriformis、E. magna、E. perforans及E. media)。以次氯酸鈉處理及溶解緩衝液作用,再以玻璃珠將已芽孢化卵囊均質化後萃取DNA。11個引子中,E113、E114及E120對E. piriformis皆可產生獨特的電泳帶,此可用於與E. magna、E. media、E. perforans區別。而E111則在E. magna、E. media及E. perforans可產生不同的電泳帶,在E. piriformis則無。因此,合併E111與E113、E114及E120的其中一個引子便可將4種艾美球蟲加以鑑別。可知,隨機增幅多型性DNA技術可用於輔助傳統型態學鑑別方法之不足。
To differentiate four Eimeria species from rabbit, namely, Eimeria piriformis, E. magna, E. perforans, and F. media, random amplified polymorphic DNA (RAPD) with 11 sets of non-specific primers, designated G2, E111 to E120, was carried out. Modification of DNA extraction from sporulated oocyst using sodium hypochlorite treatment and lysis buffer followed by glass beads homogenization was found to yield a better result. Of the 11 primer sets tested, 3 (E113, E114, and E120) were able to produce unique band patterns for E. piriformis, which can be used to differentiate it from E. magna, E. media, and E. perforans. However, primer E111 was able to produce different band patterns for E. magna, E. media, and E. perforans but no band for E. piriformis. Thus, using a combination of E111 with either E113 or E120 primers, we can differentiate each of the 4 species from one another. Thus, RAPD has the potential to be used as a molecular method for confirming and differentiating the identity of the Eimeria species from rabbit to complement the classical morphometric method.


    

本卷期目次
Taiwan Veterinary Journal 38卷4期 (2012.12)
Comparison of NP and M Primers for Avian Influenza Virus Detection by Reverse Transcription Polymerase Chain Reaction/ Hue-Wen HongIvan-Chen ChengHui-Wen ChenChing-Ho Wang
Case Report: Canine Urethral Trauma Caused by Inappropriate Instrumentation of Lithotripsy/ Yung-Chi WuShih-Chieh ChangWei-Ming LeeJen-Tzu YangJih-Jung LeePei-Yen ChihHsien-Chi Wang
Differentiation of Four Eimeria Species Commonly Seen in Taiwanese Rabbits by RAPD/ Ming-Hsien LiChao-Tsai LiaoWei-Chih OuPei-Lain ChenChiung-Hua HuangYu-Hsuan ChenHai-I HuangHong-Kean Ooi
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