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1
題名:Photosensitizing Capabilities of Photofrin in Vascular Endothelial Cells     (14點)
Photofrin光顯劑於血管顯微內皮細胞及惡性病變光顯能力之評估
著者:Cheng-Jen Chang(張承仁) ;Shwu-Fan Ma(馬淑芳) ;Fu-Chan Wei(魏福全)
出版地區:台灣
出版城市:台北市
學科:醫學綜合
關鍵字:Photofrin ; microvascular endothelial cell ; chicken combs
刊名:Formosan journal of surgery
卷期:34卷6期(2001.12)
頁碼:287-293
語言:英語
摘要: 英文摘要PDF

The object of the present study was to evaluate the feasibility of photodynamic therapy (PDT) using Photofrin@ (porfimer sodium), an United States Food and Drug Administration approved photosensitizer. The in vitro absorption and photosensitizing activities of Photofrin@ were evaluated with cultured human microvascular endothelial cells and in vivo activities were evaluated with ductal carcinoma cell stimulated mammary tumor. The viability of microvascular endothelial cells was assessed using rhodamine-phallodin coupled α-tubulin antibody specific for cytoplasmic microtubule and visualized by immunofluorescence microscopy. At the optimal wavelength of 630 nm, the percentage of microvascular endothelial cells killed by variable concentrations of Photofrin@ was measured by either live/dead or lactate dehydrogenase-released assays and the in vivo biological activity was assessed by determining the amount of necrosis produced in stimulated mammary tumor. A combination of 3.5μ g/mL Photofrin@ and laser light at 630 nm with a power density of 100 m W Icm2 resulted in a 50 % cell kill. The lactate dehydrogenase released from microvascular endothelial cells after PDT showed a 50% optical density obtained from each concentration of photosensitizer of 50% cell killing after laser illumination. Stained histology specimens of stimulated mammary tumor demonstrated significant vascular destruction 1 hour after PDT. In conclusion, Photofrin@ has the capabilities to destroy microvascular endothelial cells in vitro and vasculature in vivo. However, the degree of absorption and tissue destruction in vasculature in vivo and different anatomical structures should be considered and will be included in our subsequent studies.


    

本卷期目次
Formosan journal of surgery 34卷6期 (2001.12)
Recent Advances in the Surgical Treatment of Primary Hyperparathyroidism/ Chen-Hsen Lee
Photosensitizing Capabilities of Photofrin in Vascular Endothelial Cells/ Cheng-Jen ChangShwu-Fan MaFu-Chan Wei
Efficacy of Autologous Percutaneous Bone Marrow Graft Injection for Treatment of Nonunion of the Tibia/ Jiann-Long JeanHsiou-Lan TangShyu-Jye WangJhi-Hong ChangSung-Chun LinChih-Ju ChenChi-Jen YangTai-Chang ChernHsin-Pai LeeChian-Her Lee
Treatment of Transverse Patella Fractures with Cannulated Screws and Tension Band Fixation/ Hao-Chen LiuJun-Wen WangFong-Chi Chen
Autoamputation Following Transendoscopic Sympathectomies for Peripheral Arterial Occlusion/ Chung-Nan LinYu-Chun WuNai-Phon Wang
Intraosseous Ganglion Cyst of the Distal Femur--A Case Report/ Ming-Hung ChenChang-Hwa TuKuo-Chung HsiaoJiann-Shenq JouMing-Hsien Lin
Teratoma of the Low Brain Stem--A Case Report/ Cheng-Chia TsaiFu-Chao Huang
Primary Carcinoid Thmor of the Gallbladder--A Case Report and Review of the Literature/ Heng-Hui LienFeng-Chuan TaiChing-Shui HuangKai-Mo ChenTsung-Hsi Wang
Rectal Stenosis--An Unusual Presentation of Prostate Adenocarcinoma/ Kuang-Yi LiuJeng-Kae JiangJen-Kou Lin
Radical Excision of Malignant Thymoma with Invasion of the Superior Vena Cava and Right Atrium--A Case Report/ Chao-Jan YangJiun-Yi Li
 
   
 
   

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