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題名:己內醯胺脫氮菌之分離、脫氮情形與生理特性     (20點)
Isolation, Denitrification and Physiological Characterization of ε-Caprolactam Denitrifying Bacteria
著者:王俊欽(Chun-Chin Wang) ;李季眉(Chi-Mei Lee)
出版地區:台灣
出版城市:彰化縣
學科:自科綜合 ; 應科綜合
關鍵字:生物分解 ; 己內醯胺 ; 脫氮 ; biodegradation ; ε-caprolactam ; denitrification ; Paracoccus versutus
刊名:科學與工程技術期刊
卷期:3卷1期(2007.3)
頁碼:59-68
語言:繁體中文
摘要: 中文摘要PDF ; 英文摘要PDF

己內醯胺?工業上常用之化學物質,若未妥善處理此類廢水即將其排入天然水體中,對整個水體生態及人類生活品質有很大之衝擊與傷害。本研究嘗試從丙烯?-苯乙烯-丁二烯(acrylonitrile-butadiene-styrene, ABS)樹脂製造廠與聚丙烯?(polyacrylonitrile, PAN)人造纖維製造廠中,分離及篩選出能利用己內醯胺脫氮之菌株,且瞭解實廠污泥及篩選所得的菌株以人工合成廢水中己內醯胺脫氮的情形。並使用分子生物技術,獲得己內醯胺脫氮菌的16S rDNA序列,配合網站搜尋到其他脫氮菌的16S rDNA序列,將不同菌株之序列加以比對後,希望得知己內醯胺脫氮菌與其他脫氮菌間的親緣關係。實驗結果顯示:不論ABS混合族群或PAN混合族群皆可利用1538.5mg/l以下之己內醯胺進行脫氮,且適量之電子接受者是必要的。菌株Paracoccus versutus MDC-3與Paracoccus versutus TDC-2分別來自ABS樹脂製造廠與PAN人造纖維製造廠,該二株菌皆可利用1445.8mg/l以下之己內醯胺脫氮,?使己內醯胺完全去除,適量之硝酸鹽氮是必要的。菌株P. versutus MDC-3、P. versutus TDC-2 與Hyphomicrobium sp. HM(甲醇脫氮菌)、Methylosinus pucelana(甲烷脫氮菌)、Magnetospirillum sp. CC-26(酚脫氮菌)之親緣關係較近,而與其他脫氮菌之關係較遠。
The chemical compound ε-caprolactam is widely used in industry. Due to its carcinogenicity and toxicity, discharge of this chemical compound into natural water and soil systems may lead to an adverse environmental impact on water quality, thus endangering public health and welfare. This study attempted to isolate and identify the denitrifying bacteria from an acrylonitrile-butadiene-styrene (ABS) resin-manufactured wastewater treatment system and a polyacrylonitrile (PAN) fiber-manufactured wastewater treatment system. These bacteria can utilize ε-caprolactam for denitrification. Another aim of this study was to understand the function of isolated pure strains and mixed bacteria cultures in treating ε-caprolactam from synthetic wastewater. Finally, phylogenetic trees were generated to understand the relationships between bacteria which can utilize ε-caprolactam for denitrification by methods based on the 16S rDNA gene sequence. The results indicated that both ABS and PAN mixed bacteria cultures can utilize ε-caprolactam up to 1538.5 mg/l for denitrification from synthetic wastewater. Moreover, a sufficient supply of a nitrate electron acceptor was necessary for the complete removal of ε-caprolactam. Paracoccus versutus MDC-3 and Paracoccus versutus TDC-2 strains were isolated from the ABS resin-manufactured wastewater treatment system and the PAN fiber-manufactured wastewater treatment system, respectively. Both strains were able to utilize ε-caprolactam up to 1445.8mg/l for denitrification, a sufficient supply of nitrate being necessary for the complete removal of ε-caprolactam. The strains P. versutus MDC-3, P. versutus TDC-2, Hyphomicrobium sp. HM (methanol denitrifying bacteria), Methylosinus pucelana (methane denitrifying bacteria) and Magentospirillum sp. CC-26 (phenol denitrifying bacteria) had intimate relationships on the basis of the phylogenetic trees.


    

本卷期目次
科學與工程技術期刊 3卷1期 (2007.3)
電腦輔助工程在CNC立式加工中心機進給系統高速化之應用/ 陳俊達王焜潔周信暐
三維重疊式網格數值模擬法之建立/ 秦雅嫺
己內醯胺脫氮菌之分離、脫氮情形與生理特性/ 王俊欽李季眉
Using an N-gram-Based Mapping Approach to Content-Based Music Information Retrieval/ Chueh-Chih LiuTe-Wei ChiangTien-Wei Tsai
MC-CDMA系統工作於存在高斯相關分支與選頻性通道環境中的效能分析/ 陳雍宗劉昱吟
3吋小型液晶面板LED背光系統之薄型導光板設計研究/ 黃顯川楊萬隆葉志庭
對向汽車頭燈照射對正向駕駛者辨識距離的影響/ 楊旻洲王凱正
偏壓輔助成核對多晶CVD鑽石成長之影響/ 李世鴻焦繼葳
利用共生詞彙特性發展一個二階段文件群集法/ 李維平吳澤民王美淳
偏壓輔助成核對多晶CVD鑽石成長之影響/ 李世鴻焦繼葳
對向汽車頭燈照射對正向駕駛者辨識距離的影響/ 楊旻洲王凱正
MC-CDMA系統工作於存在高斯相關分支與選頻性通道環境中的效能分析/ 陳雍宗劉昱吟
三維重疊式網格數值模擬法之建立/ 秦雅嫺
Using an N-gram-Based Mapping Approach to Content-Based Music Information Retrieval/ Chueh-Chih LiuTe-Wei ChiangTien-Wei Tsai
己內醯胺脫氮菌之分離、脫氮情形與生理特性/ 王俊欽李季眉
The Effects of Cutoff Voltage and Heat Treatment on the Behavior of a Nanocrystalline Cu-Doped Tin Oxide Applied to a Li-Ion Battery/ Shao-Ting ChangIng-Chi LeuMin-Hsiung Hon
電腦輔助工程在CNC立式加工中心機進給系統高速化之應用/ 陳俊達王焜潔周信暐
3吋小型液晶面板LED背光系統之薄型導光板設計研究/ 黃顯川楊萬隆葉志庭
利用共生詞彙特性發展一個二階段文件群集法/ 李維平吳澤民王美淳
 
   
 
   

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